Stem cell differentiation12/10/2023 Dedifferentiation, transdifferentiation and reprogramming: three routes to regeneration. Derivation of novel human ground state naive pluripotent stem cells. Reorganization of enhancer patterns in transition from naive to primed pluripotency. In this study, the authors used whole-genome bisulfite sequencing to show that 2i-cultured ESCs and pre-implantation mouse epiblasts have low levels of DNA methylation, and differ from serum-cultured ESCs and post-implantation embryos.īuecker, C. Whole-genome bisulfite sequencing of two distinct interconvertible DNA methylomes of mouse embryonic stem cells. The transcriptional and epigenomic foundations of ground state pluripotency. DNA methylation: roles in mammalian development. Regulatory principles of pluripotency: from the ground state up. Mechanism and reconstitution in vitro of germ cell development in mammals. References 7 and 8 describe the derivation of self-renewing ESCs from post-implantation epiblasts. Derivation of pluripotent epiblast stem cells from mammalian embryos. New cell lines from mouse epiblast share defining features with human embryonic stem cells. Dynamic stem cell states: naive to primed pluripotency in rodents and humans. Weinberger, L., Ayyash, M., Novershtern, N. Specification and epigenetic programming of the human germ line. W., Kobayashi, T., Irie, N., Dietmann, S. Zygotic genome activation during the maternal-to-zygotic transition. The molecular hallmarks of epigenetic control. Bivalency has a prominent role in post-implantation embryonic development.ĭNA methylation and H3K27me3 are mutually exclusive at CpG-rich promoters: DNA methylation prevents deposition of the H3K27me3 mark.Įxit from naive pluripotency and embryonic stem cell differentiation is accompanied by progressive restriction of chromatin accessibility and histone acetylation. H3K27me3 and H3K4me3 promoter bivalency is observed in stem cells and in differentiated cells. Topologically associated domains (TADs) are largely stable during stem cell differentiation but sub-TAD interactions might change.Įnhancer activation is initiated by pioneer transcription factors and is often followed by H3K4me1 deposition (enhancer priming) and H3K27 acetylation (enhancer activation). Repressive epigenetic marks (such as DNA methylation, dimethylation of histone H3 at lysine 9 (H3K9me2) and H3K27me3) are dispensable for pre-implantation embryonic development and naive pluripotency.ĭNA methylation patterns change through development: naive pluripotency, pre-implantation epiblasts and primordial germ cells are associated with global DNA demethylation, whereas post-implantation epiblasts and epiblast-derived stem cells (EpiSCs) have high levels of DNA methylation.ĭynamic chromatin interactions occur during development and stem cell differentiation.
0 Comments
Leave a Reply.AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |